Collision between a FlxTilemap and at least one FlxObject is really simple. Just call FlxG.collide(), passing the tilemap and the object(s), and flixel takes care of the rest.

FlxG.collide(map, sprite);

Demonstration

package;

import flixel.FlxG;
import flixel.FlxSprite;
import flixel.FlxState;
import flixel.tile.FlxTilemap;

class PlayState extends FlxState
{
	var map:FlxTilemap;
	var sprite:FlxSprite;
	
	override function create()
	{
		super.create();
		
		map = new FlxTilemap();
		map.loadMapFromCSV("assets/room-map.csv", "assets/tiles.png", 16, 16);
		add(map);
		
		sprite = new FlxSprite("assets/sprite.png");
		sprite.screenCenter();
		sprite.velocity.set(200, 200);
		sprite.elasticity = 1;
		add(sprite);
	}
	
	override function update(elapsed:Float)
	{
		super.update(elapsed);
		
		FlxG.collide(map, sprite);
	}
}